Journal: Indian Journal of Experimental Biology

Article Title: Growth factors and culture media dependent in vitro expansion and characteristics of enriched spermatogonial stem cells derived from adult caprine testis

doi: 10.56042/ijeb.v63i05.15249

Figure Lengend Snippet: Fig. 6 — Comparative evaluation of the effect of culture media on the cluster-forming activity (CFA) of cSScs. (A) The enriched population of cSSCs were grown in different culture media (In-house, MEM, MesenPRO, StemPRO, Stemline, and Nutrient F12 media) for 7 days. After incubation for 7 days, the cell clusters were washed with 1× PBS and fixed with 4% paraformaldehyde for 15 minutes at RT. Following fixation, the clusters were stained with 1% crystal violet solution for 30 minutes at RT. After washing, the clusters were examined under an inverted microscope. (B) Comparison of optical densities showing the effect of different culture media on CFA efficiency of cSSCs. Optical density was measured after solubilization of stain (crystal violet) with 1% SDS solution.

Article Snippet: The stained cell colonies were then washed carefully with distilled water before examined under an upright microscope (Motic; BA410E) equipped with a digital camera (Moticam 4000X).

Techniques: Activity Assay, Incubation, Staining, Inverted Microscopy, Comparison